Immunophenotyping and cytogenetic profile of children with acute lymphoblastic leukemia: A study from a tertiary care center from Kashmir.
Abstract
Background
The complete cytogenetic and immunophenotyping data in children suffering from acute lymphoblastic leukemia (ALL) in Jammu and Kashmir is scarce. To bridge this knowledge gap the present study proposes to evaluate the immunophenotype and cytogenetic profile of pediatric ALL patients treated in our hospital.
Material and methods
This hospital-based observational study was conducted on 180 pediatric patients aged between 1 to 18 years who had visited the Paediatric unit of the Department of Medical Oncology at Sher-I -Kashmir Institute of Medical Science, Srinagar ,Jammu and Kashmir between the January 2015 to December 2019.
Result
Among the study participants, 57.8% were male and 42.2% were female with a mean age of 9.24 years and median of 8 Years. Among the participants, 57.2% were below 10 years of age and 42.8% were above 10years of age. CNS disease was reported in 7.8% of the study participants. 63.3% patients had a TLC count of less than 20000. Immunophenotyping data revealed pre-B ALL in 77.8% of children. Cytogenetic study was conducted on 153 patients among them 74.4% had a normal karyotype, 7.2% s had hyperdiploidy and 3.3% had hypodiploidy. The FISH analysis showed that 23.3% of study participants were positive for the TEL-AML study, 11.1% were positive for BCR-ABL analysis and 4.4% of participants were positive for MLL gene analysis. The overall survival in the study population was 78.9% among the study participants. Only the MLL gene rearrangement analysis showed a statistically significant correlation with the survival analysis (P<0.5).
Conclusion
In summary, the present study reported the complete cytogenetic and immunophenotyping profile of the children suffering from acute lymphoblastic leukemia in Jammu and Kashmir.
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References
2. Kumar, D., Panigrahi, M. K., Saikia, K. K., Kapoor, G. & Mehta, A. Molecular analysis of childhood B-acute lymphoblastic leukemia: Identification and prognosis of rare breakpoints. Mol. Biol. 49, 843–847 (2015).
3. PDQ Pediatric Treatment Editorial Board. Childhood Acute Lymphoblastic Leukemia Treatment (PDQ®): Health Professional Version. in PDQ Cancer Information Summaries (National Cancer Institute (US), 2002).
4. Noronha, E. P. et al. The Profile of Immunophenotype and Genotype Aberrations in Subsets of Pediatric T-Cell Acute Lymphoblastic Leukemia. Front. Oncol. 9, 316 (2019).
5. De Marchi, F., Araki, M. & Komatsu, N. Molecular features, prognosis, and novel treatment options for pediatric acute megakaryoblastic leukemia. Expert Rev. Hematol. 12, 285–293 (2019).
6. Siddaiahgari, S., Awaghad, M. & Latha, M. Clinical, immunophenotype and cytogenetic profile of acute lymphoblastic leukemia in children at tertiary health care centre in India. Muller J. Med. Sci. Res. 6, 112 (2015).
7. Padhi, S. et al. Cytogenetic profile of pediatric acute lymphoblastic leukemia (ALL): analysis of 31 cases with review of literature. Caryologia 64, 33–41 (2011).
8. Khalid, S., Moiz, B., Adil, S. N. & Khurshid, M. Retrospective review of pediatric patients with acute lymphoblastic leukemia: a single center experience. Indian J. Pathol. Microbiol. 53, 704 (2010).
9. Alkhayat, N. et al. Cytogenetic Profile and FLT3 Gene Mutations of Childhood Acute Lymphoblastic Leukemia. Clin. Med. Insights Oncol. 11, 117955491772171 (2017).
10. Gupta, N. et al. FxCycleTM Based Ploidy Correlates with Cytogenetic Ploidy in B-Cell Acute Lymphoblastic Leukemia and Is Able to Detect the Aneuploid Minimal Residual Disease Clone. Cytometry B Clin. Cytom. 96, 359–367 (2019).
11. Wang, Y. M., Mo, J. Q., Kuo, D. J. & Wong, V. MLL rearranged acute lymphoblastic leukaemia presenting as a maxillary sinus mass with a discordant immunophenotypic profile from the bone marrow. BMJ Case Rep. CP 12, (2019).
12. Smith, M. C., Kressin, M. K., Crawford, E., Wang, X. J. & Kim, A. S. B Lymphoblastic Leukemia With a Novel t(11;15) (q23;q15) and Unique Burkittoid Morphologic and Immunophenotypic Findings in a 9-Year-Old Boy. Lab. Med. 46, 320–326 (2015).
13. Amare, P. S. K. et al. Cytogenetic Profile in 7209 Indian Patients with de novo Acute Leukemia: A Single Centre Study from India. J. Cancer Ther. 7, 530–544 (2016).
